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Serum amyloid P component Recombinant antibody

Serum amyloid P component Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241403F5

Cat no : 84402-1-RR

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Serum amyloid P component Recombinant antibody (84402-1-RR)

    WB analysis of human plasma using 84402-1-RR

    human plasma was subjected to SDS PAGE followed by western blot with 84402-1-RR (Serum amyloid P component antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84402-1-RR

    Affinity and Kinetic Characterization of 84402-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84402-1-RR against Human Serum amyloid P component were performed. The affinity constant is 1.49 nM.

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84402-1-RR is Serum amyloid P component Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name amyloid P component, serum
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC007058
Gene Symbol Serum amyloid P component
Gene ID (NCBI) 325
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Protocols

Product Specific Protocols
WB protocol for Serum amyloid P component antibody 84402-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols