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  • Western Blot (WB) analysis of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    WB analysis of mouse spleen using 84365-5-RR

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    IHC staining of mouse spleen using 84365-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using SIGNR1/CD209b Recombinant antibody (84365-5-RR)

    IHC staining of mouse spleen using 84365-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84365-5-RR (SIGNR1/CD209b antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84365-5-RR

    Affinity and Kinetic Characterization of 84365-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84365-5-RR against Mouse SIGNR1/CD209b were performed. The affinity constant is 0.155 nM.

SIGNR1/CD209b Recombinant antibody

SIGNR1/CD209b Recombinant Antibody for WB, IHC, ELISA
Cat No. 84365-5-RR
Clone No.241726B1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

DC-SIGN-related protein 1, DC-SIGNR1, Cd209b, CD209 antigen-like protein B, CD209

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84365-5-RR targets SIGNR1/CD209b in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein Predict reactive species
Full Name CD209b antigen
Calculated Molecular Weight37 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberNM_026972.5
Gene Symbol Cd209b
Gene ID (NCBI) 69165
RRIDAB_3671901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8CJ91-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIGNR1 (CD209b) is a mouse homolog of the human DC-SIGN receptor (PMID: 11581173). SIGNR1 is a type II transmembrane protein with an extracellular domain consisting of a neck region made up of four repeats of 28 amino acids and a carbohydrate recognition domain (PMID: 15583012). It is expressed on marginal zone macrophages and peritoneal macrophages and is a key receptor for the recognition and efficient clearance of pathogens by the innate immune system.

Protocols

Product Specific Protocols
WB protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
IHC protocol for SIGNR1/CD209b antibody 84365-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols