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CLUH Recombinant antibody

CLUH Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241640C11

Cat no : 84321-4-RR

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLUH Recombinant antibody (84321-4-RR)

    IF Staining of HeLa using 84321-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLUH antibody (84321-4-RR, Clone: 241640C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84321-4-RR is CLUH Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLUH fusion protein Ag34275
Full Name KIAA0664
GenBank Accession NumberBC156940
Gene Symbol CLUH
Gene ID (NCBI) 23277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)

Protocols

Product Specific Protocols
IF protocol for CLUH antibody 84321-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols