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Ch-TOG Recombinant antibody, PBS Only (Detector)

Ch-TOG Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241546G8

Cat no : 84285-3-PBS

Synonyms

CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ch-TOG Recombinant antibody (84285-3-RR)

    WB analysis using 84285-3-RR (same clone as 84285-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84285-3-RR (Ch-TOG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Ch-TOG Recombinant antibody (84285-3-RR)

    FC experiment of HeLa using 84285-3-RR (same clone as 84285-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ckap5 Recombinant Antibody (84285-3-RR, Clone:241546G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using Ch-TOG Recombinant antibody (84285-3-RR)

    FC experiment of A549 using 84285-3-RR (same clone as 84285-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Ckap5 Recombinant Antibody (84285-3-RR, Clone:241546G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01203-1

    Cytometric bead array standard curve of MP01203-1

    Cytometric bead array standard curve of MP01203-1, Ch-TOG Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84285-1-PBS. Detection antibody: 84285-3-PBS. Standard: Ag24875. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 84285-3-RR

    Affinity and Kinetic Characterization of 84285-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84285-3-RR against Human Ch-TOG were performed. The affinity constant is 1.05 nM.

Product Information

84285-3-PBS targets Ch-TOG as part of a matched antibody pair:

MP01203-1: 84285-1-PBS capture and 84285-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ch-TOG fusion protein Ag24875
Full Name cytoskeleton associated protein 5
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC120869
Gene Symbol Ch-TOG
Gene ID (NCBI) 9793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.