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CPN1 Recombinant antibody, PBS Only (Detector)

CPN1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241481A5

Cat no : 84218-1-PBS

Synonyms

Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase, ACBP

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using CPN1 Recombinant antibody (84218-1-RR)

    WB analysis of human plasma using 84218-1-RR (same clone as 84218-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84218-1-RR (CPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of rat liver using 84218-1-RR (same clone as 84218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of rat liver using 84218-1-RR (same clone as 84218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IF Staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CPN1 antibody (84218-1-RR, Clone: 241481A5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01152-1

    Cytometric bead array standard curve of MP01152-1

    Cytometric bead array standard curve of MP01152-1, CPN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84218-2-PBS. Detection antibody: 84218-1-PBS. Standard: Ag4035. Range: 0.781-100 ng/mL.

Product Information

84218-1-PBS targets CPN1 as part of a matched antibody pair:

MP01152-1: 84218-2-PBS capture and 84218-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPN1 fusion protein Ag4035
Full Name carboxypeptidase N, polypeptide 1
Calculated Molecular Weight 458 aa, 52 kDa
Observed Molecular Weight40~50 kDa
GenBank Accession NumberBC027897
Gene Symbol CPN1
Gene ID (NCBI) 1369
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.