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CPN1 Recombinant antibody

CPN1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241481A5

Cat no : 84218-1-RR

Synonyms

Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase, ACBP

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using CPN1 Recombinant antibody (84218-1-RR)

    WB analysis of human plasma using 84218-1-RR

    human plasma were subjected to SDS PAGE followed by western blot with 84218-1-RR (CPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of mouse liver using 84218-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of rat liver using 84218-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of rat liver using 84218-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IHC staining of mouse liver using 84218-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CPN1 Recombinant antibody (84218-1-RR)

    IF Staining of mouse liver using 84218-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CPN1 antibody (84218-1-RR, Clone: 241481A5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inmouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84218-1-RR is CPN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPN1 fusion protein Ag4035
Full Name carboxypeptidase N, polypeptide 1
Calculated Molecular Weight 458 aa, 52 kDa
Observed Molecular Weight40~50 kDa
GenBank Accession NumberBC027897
Gene Symbol CPN1
Gene ID (NCBI) 1369
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.

Protocols

Product Specific Protocols
WB protocol for CPN1 antibody 84218-1-RRDownload protocol
IHC protocol for CPN1 antibody 84218-1-RRDownload protocol
IF protocol for CPN1 antibody 84218-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols