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NTB-A/SLAMF6 Recombinant antibody

NTB-A/SLAMF6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241384G5

Cat no : 84211-4-RR

Synonyms

SLAMF6, Ly108, KALIb, KALI, CD352

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NTB-A/SLAMF6 Recombinant antibody (84211-4-RR)

    WB analysis using 84211-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84211-4-RR (NTB-A/SLAMF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using NTB-A/SLAMF6 Recombinant antibody (84211-4-RR)

    IF Staining of Ramos using 84211-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using NTB-A/SLAMF6 antibody (84211-4-RR, Clone: 241384G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84211-4-RR

    Affinity and Kinetic Characterization of 84211-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84211-4-RR against Human NTB-A/SLAMF6 were performed. The affinity constant is 2.73 nM.

Tested Applications

Positive WB detected inDaudi cells, Jurkat cells
Positive IF/ICC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84211-4-RR is NTB-A/SLAMF6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name SLAM family member 6
Calculated Molecular Weight37kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_001184714.2
Gene Symbol SLAMF6
Gene ID (NCBI) 114836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).

Protocols

Product Specific Protocols
WB protocol for NTB-A/SLAMF6 antibody 84211-4-RRDownload protocol
IF protocol for NTB-A/SLAMF6 antibody 84211-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols