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METTL6 Recombinant antibody

METTL6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241482F12

Cat no : 84209-4-RR

Synonyms

tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL6 Recombinant antibody (84209-4-RR)

    WB analysis using 84209-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84209-4-RR (METTL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84209-4-RR

    Affinity and Kinetic Characterization of 84209-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84209-4-RR against Human METTL6 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84209-4-RR is METTL6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL6 fusion protein Ag9714
Full Name methyltransferase like 6
Calculated Molecular Weight 255 aa, 30 kDa
Observed Molecular Weight 30-32 kDa
GenBank Accession NumberBC022400
Gene Symbol METTL6
Gene ID (NCBI) 131965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non-tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

Protocols

Product Specific Protocols
WB protocol for METTL6 antibody 84209-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols