Filter: AllWBIF/ICCFC (Intra)Cytometric bead arrayAffinity

WB analysis of HeLa using 84207-1-RR (same clone as 84207-1-PBS)
WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
WB analysis using 84207-1-RR (same clone as 84207-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 84207-1-RR (same clone as 84207-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 84207-1-RR (same clone as 84207-1-PBS)
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
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1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 84207-1-RR (same clone as 84207-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84207-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01139-1
Cytometric bead array standard curve of MP01139-1, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-2-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.
Cytometric bead array standard curve of MP01139-2
Cytometric bead array standard curve of MP01139-2, HNRNPAB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84207-1-PBS. Detection antibody: 84207-3-PBS. Standard: Ag35010. Range: 0.625-80 ng/mL.
Affinity and Kinetic Characterization of 84207-1-PBS
Biolayer interferometry (BLl) kinetic assay of 84207-1-PBS against Human HNRNPAB was performed. The affinity constant is 2.01 nM.
Affinity and Kinetic Characterization of 84207-1-RR
Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

Featured Product
KD/KO Validated

HNRNPAB Recombinant antibody, PBS Only (Capture)

HNRNPAB {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84207-1-PBS

Clone No.241462B1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

241462B1, ABBP 1, ABBP1, ABBP-1, APOBEC1-binding protein 1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84207-1-PBS targets HNRNPAB as part of a matched antibody pair:

MP01139-1: 84207-1-PBS capture and 84207-2-PBS detection (validated in Cytometric bead array)

MP01139-2: 84207-1-PBS capture and 84207-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	HNRNPAB fusion protein Ag35010 Predict reactive species
Full Name	heterogeneous nuclear ribonucleoprotein A/B
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36-42 kDa
GenBank Accession Number	BC036708
Gene Symbol	HNRNPAB
Gene ID (NCBI)	3182
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q99729
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).