Filter: AllWBIF/ICCFC (Intra)Affinity

WB analysis of HeLa using 84207-1-RR
WB result of HNRNPAB antibody (84207-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPAB transfected HeLa cells.
WB analysis using 84207-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84207-1-RR (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 84207-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
IF Staining of HeLa using 84207-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (84207-1-RR, Clone: 241462B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of Jurkat using 84207-1-RR
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
FC experiment of HeLa using 84207-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPAB Recombinant antibody (84207-1-RR, Clone:241462B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Affinity and Kinetic Characterization of 84207-1-RR
Biolayer interferometry (BLl) kinetic assays of 84207-1-RR against Human HNRNPAB were performed. The affinity constant is 1.07 nM.

Featured Product
KD/KO Validated

HNRNPAB Recombinant antibody

HNRNPAB {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84207-1-RR

Clone No.241462B1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1, ABBP 1

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, HepG2 cells, HEK-293T cells, K-562 cells
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	Jurkat cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84207-1-RR targets HNRNPAB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	HNRNPAB fusion protein Ag35010 Predict reactive species
Full Name	heterogeneous nuclear ribonucleoprotein A/B
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36-42 kDa
GenBank Accession Number	BC036708
Gene Symbol	HNRNPAB
Gene ID (NCBI)	3182
RRID	AB_3671763
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q99729
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).