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PPID Recombinant antibody

PPID Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241361B2

Cat no : 84115-1-RR

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPID Recombinant antibody (84115-1-RR)

    WB analysis using 84115-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84115-1-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using PPID Recombinant antibody (84115-1-RR)

    FC experiment of A431 using 84115-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PPID Recombinant Antibody (84115-1-RR, Clone:241361B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84115-1-RR

    Affinity and Kinetic Characterization of 84115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84115-1-RR against Human PPID were performed. The affinity constant is 0.313 nM.

Tested Applications

Positive WB detected inA549 cells, MCF7 cells, Jurkat cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84115-1-RR targets PPID in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPID fusion protein Ag3409
Full Name peptidylprolyl isomerase D
Calculated Molecular Weight 370 aa, 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC030707
Gene Symbol PPID
Gene ID (NCBI) 5481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Protocols

Product Specific Protocols
WB protocol for PPID antibody 84115-1-RRDownload protocol
FC protocol for PPID antibody 84115-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols