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CD2 Recombinant antibody

CD2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241222B8

Cat no : 84108-5-RR

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84108-5-RR)

    WB analysis using 84108-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84108-5-RR)

    WB analysis using 84108-5-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84108-5-RR

    Affinity and Kinetic Characterization of 84108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

Tested Applications

Positive WB detected inJurkat cells, NK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84108-5-RR is CD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD2 molecule
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
WB protocol for CD2 antibody 84108-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols