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PROS1 Recombinant antibody, PBS Only (Capture)

PROS1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241241E5

Cat no : 84107-2-PBS

Synonyms

Vitamin K-dependent protein S, PROS, 241241E5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROS1 Recombinant antibody (84107-2-RR)

    WB analysis using 84107-2-RR (same clone as 84107-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01044-1

    Sandwich ELISA standard curve of MP01044-1

    Sandwich ELISA standard curve of MP01044-1, Human PROS1 Recombinant Matched Antibody Pair - PBS only. 84107-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10539. 84107-1-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL

  • Sample test of MP01044-1

    Sample test of MP01044-1

    The mean PROS1 concentration was determined to be 276.09 ng/mL in HuH-7 cell extract based on a 1.4 mg/mL extract load.

  • Flow cytometry (FC) experiment of A549 cells using PROS1 Recombinant antibody (84107-2-RR)

    FC experiment of A549 using 84107-2-RR (same clone as 84107-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01044-1

    Cytometric bead array standard curve of MP01044-1

    Cytometric bead array standard curve of MP01044-1, PROS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84107-2-PBS. Detection antibody: 84107-1-PBS. Standard: Ag10539. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84107-2-RR

    Affinity and Kinetic Characterization of 84107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

Product Information

84107-2-PBS targets PROS1 as part of a matched antibody pair:

MP01044-1: 84107-2-PBS capture and 84107-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PROS1 fusion protein Ag10539
Full Name protein S (alpha)
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC015801
Gene Symbol PROS1
Gene ID (NCBI) 5627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.