Filter:
Tested Applications
| Positive WB detected in | DU 145 cells, PC-3 cells, HuH-7 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84107-2-RR targets PROS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | PROS1 fusion protein Ag10539 Predict reactive species |
| Full Name | protein S (alpha) |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC015801 |
| Gene Symbol | PROS1 |
| Gene ID (NCBI) | 5627 |
| RRID | AB_3671673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07225 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PROS1 antibody 84107-2-RR | Download protocol |
| IHC protocol for PROS1 antibody 84107-2-RR | Download protocol |
| IF protocol for PROS1 antibody 84107-2-RR | Download protocol |
| FC protocol for PROS1 antibody 84107-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
