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TCF1/TCF7 Recombinant antibody

TCF1/TCF7 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241154D2

Cat no : 84089-4-RR

Synonyms

TCF7, TCF-1, TCF1, TCF 7, TCF 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCF7 Recombinant antibody (84089-4-RR)

    WB analysis using 84089-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84089-4-RR (TCF7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84089-4-RR is TCF1/TCF7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCF1/TCF7 fusion protein Ag5792
Full Name transcription factor 7 (T-cell specific, HMG-box)
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC048769
Gene Symbol TCF7
Gene ID (NCBI) 6932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).

Protocols

Product Specific Protocols
WB protocol for TCF1/TCF7 antibody 84089-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols