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FKBP2 Recombinant antibody

FKBP2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241166B9

Cat no : 84077-6-RR

Synonyms

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 241166B9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP2 Recombinant antibody (84077-6-RR)

    WB analysis using 84077-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84077-6-RR (FKBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84077-6-RR

    Affinity and Kinetic Characterization of 84077-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84077-6-RR against Human FKBP2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells, HuH-7 cells, NIH/3T3 cells, mouse cerebellum tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84077-6-RR is FKBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FKBP2 fusion protein Ag2255
Full Name FK506 binding protein 2, 13kDa
Calculated Molecular Weight 142 aa, 16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC003384
Gene Symbol FKBP2
Gene ID (NCBI) 2286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant (PMID:8373365).

Protocols

Product Specific Protocols
WB protocol for FKBP2 antibody 84077-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols