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AGXT2L1 Recombinant antibody

AGXT2L1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241119C4

Cat no : 83984-6-RR

Synonyms

ETNPPL, Ethanolamine-phosphate phospho-lyase, EC:4.2.3.2, Alanine--glyoxylate aminotransferase 2-like 1, 241119C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT2L1 Recombinant antibody (83984-6-RR)

    WB analysis using 83984-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83984-6-RR (AGXT2L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83984-6-RR

    Affinity and Kinetic Characterization of 83984-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83984-6-RR against Human AGXT2L1 were performed. The affinity constant is 5.17 nM.

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83984-6-RR is AGXT2L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AGXT2L1 fusion protein Ag10584
Full Name alanine-glyoxylate aminotransferase 2-like 1
Calculated Molecular Weight 499 aa, 56 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC022526
Gene Symbol AGXT2L1
Gene ID (NCBI) 64850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768).

Protocols

Product Specific Protocols
WB protocol for AGXT2L1 antibody 83984-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols