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Mouse Serpin E1/PAI-1 Recombinant antibody, PBS Only (Capture/Detector)

Serpin E1/PAI-1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241130G6

Cat no : 83980-3-PBS

Synonyms

PAI-1, Serpine1, Serpin E1, Plasminogen activator inhibitor 1, PAI1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse placenta tissue using Serpin E1/PAI-1 Recombinant antibody (83980-3-RR)

    WB analysis of mouse placenta using 83980-3-RR (same clone as 83980-3-PBS)

    mouse placenta tissue was subjected to SDS PAGE followed by western blot with 83980-3-RR (Serpin E1/PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83980-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00875-4

    Sandwich ELISA standard curve of MP00875-4

    Sandwich ELISA standard curve of MP00875-4, Mouse Serpin E1/PAI-1 Recombinant Matched Antibody Pair - PBS only. 83980-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1100. 83980-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP00875-4

    Sample test of MP00875-4

    L-929 mouse fibroblast cells (1 x 10^6 cells/mL) were cultured for 3 days in MEM supplemented with 10% equine serum and stimulated with 10 μg/mL PHA. The cell culture supernatants was assayed for mouse Serpin E1/PAI-1 and measured 41,666.9 pg/mL.

  • Sample test of MP00875-4

    Sample test of MP00875-4

    Mouse heart tissue lyaste was measured. The mouse Serpin E1/PAI-1 concentration of detected samples was determined to be 1.46 ng/mL (based on a 4.6 mg/mL extract load).

  • Sample test of MP00875-4

    Sample test of MP00875-4

    Serum of six mice was measured. The mouse Serpin E1/PAI-1 concentration of detected samples was determined to be 16,187.9 pg/mL with a range of 6,614.7 - 24,758.6 pg/mL.

  • Cytometric bead array standard curve of MP00875-1

    Cytometric bead array standard curve of MP00875-1

    Cytometric bead array standard curve of MP00875-1, MOUSE Serpin E1/PAI-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83980-3-PBS. Detection antibody: 83980-2-PBS. Standard: Eg1100. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83980-3-RR

    Affinity and Kinetic Characterization of 83980-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83980-3-RR against Mouse Serpin E1/PAI-1 were performed. The affinity constant is 0.293 nM.

Product Information

83980-3-PBS targets Serpin E1/PAI-1 as part of a matched antibody pair:

MP00875-1: 83980-3-PBS capture and 83980-2-PBS detection (validated in Cytometric bead array)

MP00875-4: 83980-2-PBS capture and 83980-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name serine (or cysteine) peptidase inhibitor, clade E, member 1
Calculated Molecular Weight45kDa
Observed Molecular Weight45-50 kDa
Gene Symbol Serpine1
Gene ID (NCBI) 18787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.