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B7H6 Recombinant antibody

B7H6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240808F7

Cat no : 83908-3-RR

Synonyms

NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7 H6, 240808F7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7H6 Recombinant antibody (83908-3-RR)

    WB analysis using 83908-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83908-3-RR (B7H6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83908-3-RR is B7H6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name hypothetical protein DKFZp686O24166
Calculated Molecular Weight51 kDa
Observed Molecular Weight75-80 kDa
GenBank Accession NumberBC136800
Gene Symbol B7H6
Gene ID (NCBI) 374383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).

Protocols

Product Specific Protocols
WB protocol for B7H6 antibody 83908-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols