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CD25/IL2RA Recombinant antibody

CD25/IL2RA Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240906A12

Cat no : 83896-1-RR

Synonyms

CD25, 240906A12, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of MJ cells using CD25/IL2RA Recombinant antibody (83896-1-RR)

    WB analysis of MJ using 83896-1-RR

    MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83896-1-RR

    Affinity and Kinetic Characterization of 83896-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

Tested Applications

Positive WB detected inMJ cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83896-1-RR is CD25/IL2RA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 2 receptor, alpha
Calculated Molecular Weight 272 aa, 31 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).

Protocols

Product Specific Protocols
WB protocol for CD25/IL2RA antibody 83896-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols