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CD25/IL2RA Recombinant antibody, PBS Only (Detector)

CD25/IL2RA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240906A12

Cat no : 83896-1-PBS

Synonyms

CD25, 240906A12, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of MJ cells using CD25/IL2RA Recombinant antibody (83896-1-RR)

    WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

    MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83896-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00837-2

    Cytometric bead array standard curve of MP00837-2

    Cytometric bead array standard curve of MP00837-2, IL2RA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83896-2-PBS. Detection antibody: 83896-1-PBS. Standard: Eg0408. Range: 2.5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83896-1-RR

    Affinity and Kinetic Characterization of 83896-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

Product Information

83896-1-PBS targets CD25/IL2RA as part of a matched antibody pair:

MP00837-2: 83896-2-PBS capture and 83896-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 2 receptor, alpha
Calculated Molecular Weight 272 aa, 31 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).