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PHF19 Recombinant antibody, PBS Only (Detector)

PHF19 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241047D7

Cat no : 83892-3-PBS

Synonyms

Polycomb like protein 3, PHD finger protein 19, PCL3, MTF2L1, hPCL3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF19 Recombinant antibody (83892-3-RR)

    WB analysis using 83892-3-RR (same clone as 83892-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83892-3-RR (PHF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83892-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PHF19 Recombinant antibody (83892-3-RR)

    FC experiment of A549 using 83892-3-RR (same clone as 83892-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PHF19 Recombinant antibody (83892-3-RR, Clone:241047D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83892-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00868-2

    Cytometric bead array standard curve of MP00868-2

    Cytometric bead array standard curve of MP00868-2, PHF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83892-5-PBS. Detection antibody: 83892-3-PBS. Standard: Ag2511. Range: 0.313-40 ng/mL

  • Affinity and Kinetic Characterization of 83892-3-RR

    Affinity and Kinetic Characterization of 83892-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83892-3-RR against Human PHF19 were performed. The affinity constant is 0.806 nM.

Product Information

83892-3-PBS targets PHF19 as part of a matched antibody pair:

MP00868-2: 83892-5-PBS capture and 83892-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PHF19 fusion protein Ag2511
Full Name PHD finger protein 19
Calculated Molecular Weight 580 aa, 63 kDa
Observed Molecular Weight62-66 kDa
GenBank Accession NumberBC022374
Gene Symbol PHF19
Gene ID (NCBI) 26147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.