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PHF19 Recombinant antibody

PHF19 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241047D7

Cat no : 83892-3-RR

Synonyms

Polycomb like protein 3, PHD finger protein 19, PCL3, MTF2L1, hPCL3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF19 Recombinant antibody (83892-3-RR)

    WB analysis using 83892-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83892-3-RR (PHF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using PHF19 Recombinant antibody (83892-3-RR)

    FC experiment of A549 using 83892-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PHF19 Recombinant antibody (83892-3-RR, Clone:241047D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83892-3-RR

    Affinity and Kinetic Characterization of 83892-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83892-3-RR against Human PHF19 were performed. The affinity constant is 0.806 nM.

Tested Applications

Positive WB detected inA375 cells, HeLa cells, HEK-293T cells, NCCIT cells, U-251 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83892-3-RR is PHF19 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PHF19 fusion protein Ag2511
Full Name PHD finger protein 19
Calculated Molecular Weight 580 aa, 63 kDa
Observed Molecular Weight62-66 kDa
GenBank Accession NumberBC022374
Gene Symbol PHF19
Gene ID (NCBI) 26147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.

Protocols

Product Specific Protocols
WB protocol for PHF19 antibody 83892-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols