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ETV4 Recombinant antibody

ETV4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241042E10

Cat no : 83891-6-RR

Synonyms

PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ETV4 Recombinant antibody (83891-6-RR)

    WB analysis of K-562 using 83891-6-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83891-6-RR (ETV4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ETV4 Recombinant antibody (83891-6-RR)

    IF Staining of A431 using 83891-6-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETV4 antibody (83891-6-RR, Clone: 241042E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using ETV4 Recombinant antibody (83891-6-RR)

    FC experiment of U2OS using 83891-6-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ETV4 Recombinant antibody (83891-6-RR, Clone:241042E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83891-6-RR

    Affinity and Kinetic Characterization of 83891-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83891-6-RR against Human ETV4 were performed. The affinity constant is 0.157 nM.

Tested Applications

Positive WB detected inK-562 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83891-6-RR is ETV4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ETV4 fusion protein Ag0984
Full Name ets variant 4
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC007242
Gene Symbol ETV4
Gene ID (NCBI) 2118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

Protocols

Product Specific Protocols
WB protocol for ETV4 antibody 83891-6-RRDownload protocol
IF protocol for ETV4 antibody 83891-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols