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Ins1 Recombinant antibody

Ins1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240798G4

Cat no : 83867-1-RR

Synonyms

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    WB analysis of rat pancreas using 83867-1-RR

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 83867-1-RR (insulin1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IHC staining of mouse pancreas using 83867-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IHC staining of mouse pancreas using 83867-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IHC staining of mouse pancreas using 83867-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IF Staining of mouse pancreas using 83867-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IF Staining of mouse pancreas using 83867-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83867-1-RR

    Affinity and Kinetic Characterization of 83867-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83867-1-RR against Mouse Ins1 were performed. The affinity constant is 0.21 nM.

Tested Applications

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83867-1-RR is Ins1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ins1 fusion protein Ag29988
Full Name insulin I
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

Protocols

Product Specific Protocols
WB protocol for Ins1 antibody 83867-1-RRDownload protocol
IHC protocol for Ins1 antibody 83867-1-RRDownload protocol
IF protocol for Ins1 antibody 83867-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols