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Thyroid Hormone Receptor beta Recombinant antibody

Thyroid Hormone Receptor beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240619E7

Cat no : 83866-2-RR

Synonyms

THRB, THR1, NR1A2, ERBA2, c-erbA-beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using Thyroid Hormone Receptor beta Recombinant antibody (83866-2-RR)

    WB analysis of mouse pancreas using 83866-2-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83866-2-RR (Thyroid Hormone Receptor beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Thyroid Hormone Receptor beta Recombinant antibody (83866-2-RR)

    IF Staining of A431 using 83866-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thyroid Hormone Receptor beta antibody (83866-2-RR, Clone: 240619E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using Thyroid Hormone Receptor beta Recombinant antibody (83866-2-RR)

    FC experiment of U2OS using 83866-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thyroid Hormone Receptor beta Recombinant Antibody (83866-2-RR, Clone:240619E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83866-2-RR is Thyroid Hormone Receptor beta Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Thyroid Hormone Receptor beta fusion protein Ag16276
Full Name thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
Calculated Molecular Weight 476 aa, 54 kDa
Observed Molecular Weight53 kDa and 54 kDa
GenBank Accession NumberBC106929
Gene Symbol Thyroid Hormone Receptor beta
Gene ID (NCBI) 7068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thyroid hormone receptor beta (THRB), also known as ERBA2, NR1A2, and THR1, belongs to the nuclear hormone receptor family and NR1 subfamily. THRB is a nuclear hormone receptor that can act as a repressor or activator of transcription. Moreover, THRB is also a high-affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. THRB is the most abundant THR isoform in the liver but is also expressed in other tissues and mediates the effects of THs on hepatic carbohydrate and lipid metabolism (PMID: 34884910). THRB has 2 isoforms with the molecular mass of 53 and 54 kDa (refer to UniProt).

Protocols

Product Specific Protocols
WB protocol for Thyroid Hormone Receptor beta antibody 83866-2-RRDownload protocol
IF protocol for Thyroid Hormone Receptor beta antibody 83866-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols