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  • Immunohistochemistry (IHC) staining of mouse testis tissue using IQUB Recombinant antibody (83847-5-RR)

    IHC staining of mouse testis using 83847-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83847-5-RR (IQUB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IQUB Recombinant antibody (83847-5-RR)

    IHC staining of mouse testis using 83847-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83847-5-RR (IQUB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83847-5-RR

    Affinity and Kinetic Characterization of 83847-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83847-5-RR against Human IQUB were performed. The affinity constant is 0.144 nM.

IQUB Recombinant antibody

IQUB {ptg:Brand} Recombinant Antibody for IHC, ELISA
Cat No. 83847-5-RR
Clone No.240957A11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

IQ motif and ubiquitin-like domain-containing protein, 240957A11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83847-5-RR targets IQUB in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IQUB fusion protein Ag19507 Predict reactive species
Full Name IQ motif and ubiquitin domain containing
Calculated Molecular Weight 791 aa, 93 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC128181
Gene Symbol IQUB
Gene ID (NCBI) 154865
RRIDAB_3671433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NA54
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQUB (IQ motif and ubiquitin domain containing) was discovered in 2002,4 which was located on chromosome 7q31.32 and its encoded protein mainly contained 2 domains, ubiquitin-like domain and IQ domain. IQUB had apparently higher expression in breast cancer than that in normal tissues, suggesting that it may act an important role in the occurrence and development of breast cancer (PMID: 29968965). IQUB is a critical RS1 adaptor in mouse sperm flagella, and is critical for sperm motility and fertilization (PMID: 36417862).

Protocols

Product Specific Protocols
IHC protocol for IQUB antibody 83847-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols