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  • Western Blot (WB) analysis of HeLa cells using SLC31A1 Recombinant antibody (83844-5-RR)

    WB analysis of HeLa using 83844-5-RR

    WB result of SLC31A1 antibody (83844-5-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

  • Western Blot (WB) analysis of SW480 cells using SLC31A1 Recombinant antibody (83844-5-RR)

    WB analysis of SW480 using 83844-5-RR

    WB result of SLC31A1 antibody (83844-5-RR; 1:800; room temperature for 1.5 hours) with wild-type and SLC31A1 knockout SW480 cells.

  • Western Blot (WB) analysis of various lysates using SLC31A1 Recombinant antibody (83844-5-RR)

    WB analysis using 83844-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83844-5-RR (SLC31A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83844-5-RR

    Affinity and Kinetic Characterization of 83844-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83844-5-RR against Human SLC31A1 were performed. The affinity constant is 3.33 nM.

  • Featured Product
  • KD/KO Validated

SLC31A1 Recombinant antibody

SLC31A1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Cat No. 83844-5-RR
Clone No.240868D10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COPT1, 240868D10, CTR1, Truncated CTR1 form

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, SW480 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83844-5-RR targets SLC31A1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC31A1 fusion protein Ag26601 Predict reactive species
Full Name solute carrier family 31 (copper transporters), member 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21-23 kDa
GenBank Accession NumberBC013611
Gene Symbol SLC31A1
Gene ID (NCBI) 1317
RRIDAB_3671429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC31A1, also known as CTR1, COPT1 and solute carrier family 31 member 1, is a high-affinity copper transporter found in the cell membrane of human cells. It plays a crucial role in maintaining copper homeostasis within the body and functions as a homotrimer to influence dietary copper absorption in the cell membrane (PMID: 37853210; 28507097). SLC31A1 has three putative transmembrane domains, with an N-terminus located extracellularly and a C-terminus located intracellularly (PMID: 12827356). SLC31A1 can be detected a a 28 kDa band corresponding to glycosylated hCTR1 monomer, and a 24 kDa band that is unglycosylated hCTR1. In some membrane preparations a C-terminal degradation product of 17 kDa is also seen (PMID: 16135512). Additionally, SLC31A1 may also resolve as a 30 to 35 kDa and a 60 to 70 kDa protein on immunoblots, forming oligomers corresponding to the expected size of a homotrimeric complex (PMID: 12827356).

Protocols

Product Specific Protocols
WB protocol for SLC31A1 antibody 83844-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols