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ATOX1 Recombinant antibody

ATOX1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240679E9

Cat no : 83785-6-RR

Synonyms

ATX1, ATOX 1, Antioxidant 1, 240679E9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATOX1 Recombinant antibody (83785-6-RR)

    WB analysis using 83785-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83785-6-RR (ATOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-3 cells using ATOX1 Recombinant antibody (83785-6-RR)

    FC experiment of PC-3 using 83785-6-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using ATOX1 Recombinant antibody (83785-6-RR)

    FC experiment of HeLa using 83785-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83785-6-RR

    Affinity and Kinetic Characterization of 83785-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83785-6-RR against Human ATOX1 were performed. The affinity constant is 183 nM.

Tested Applications

Positive WB detected inA375 cells, A549 cells, HEK-293 cells, NCI-H1299 cells, HepG2 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83785-6-RR is ATOX1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATOX1 fusion protein Ag18460
Full Name ATX1 antioxidant protein 1 homolog (yeast)
Calculated Molecular Weight 68 aa, 8 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC112248
Gene Symbol ATOX1
Gene ID (NCBI) 475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).

Protocols

Product Specific Protocols
WB protocol for ATOX1 antibody 83785-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols