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SETDB1 Recombinant antibody

SETDB1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240805F2

Cat no : 83772-6-RR

Synonyms

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SETDB1 Recombinant antibody (83772-6-RR)

    IF Staining of HepG2 using 83772-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETDB1 antibody (83772-6-RR, Clone: 240805F2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SETDB1 Recombinant antibody (83772-6-RR)

    FC experiment of HeLa using 83772-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SETDB1 Recombinant antibody (83772-6-RR, Clone:240805F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83772-6-RR

    Affinity and Kinetic Characterization of 83772-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83772-6-RR against Human SETDB1 were performed. The affinity constant is 0.18 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83772-6-RR is SETDB1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SETDB1 fusion protein Ag1725
Full Name SET domain, bifurcated 1
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

Protocols

Product Specific Protocols
IF protocol for SETDB1 antibody 83772-6-RRDownload protocol
FC protocol for SETDB1 antibody 83772-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols