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Cathepsin D Recombinant antibody

Cathepsin D Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240775E7

Cat no : 83743-2-RR

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cathepsin D Recombinant antibody (83743-2-RR)

    WB analysis using 83743-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cathepsin D Recombinant antibody (83743-2-RR)

    WB analysis of MCF-7 using 83743-2-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using Cathepsin D Recombinant antibody (83743-2-RR)

    FC experiment of MCF-7 using 83743-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cathepsin D Recombinant antibody (83743-2-RR, Clone:240775E7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, SH-SY5Y cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83743-2-RR is Cathepsin D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name cathepsin D
Calculated Molecular Weight45kDa
Observed Molecular Weight 32 kDa, 48 kDa, 52 kDa
GenBank Accession NumberNM_001909.5
Gene Symbol Cathepsin D
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

Protocols

Product Specific Protocols
WB protocol for Cathepsin D antibody 83743-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols