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TNF-alpha Recombinant antibody

TNF-alpha Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240668B4

Cat no : 83721-7-RR

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using TNF-alpha Recombinant antibody (83721-7-RR)

    WB analysis of THP-1 using 83721-7-RR

    Untreated, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83721-7-RR (TNF-alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83721-7-RR

    Affinity and Kinetic Characterization of 83721-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83721-7-RR against Human TNF-alpha were performed. The affinity constant is 8.51 nM.

Tested Applications

Positive WB detected inTHP-1 cells, LPS treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83721-7-RR is TNF-alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated Molecular Weight26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberNM_000594.4
Gene Symbol TNF alpha
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protocols

Product Specific Protocols
WB protocol for TNF-alpha antibody 83721-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols