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MARS Recombinant antibody, PBS Only (Capture)

MARS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240648G4

Cat no : 83690-4-PBS

Synonyms

MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MARS Recombinant antibody (83690-4-RR)

    WB analysis using 83690-4-RR (same clone as 83690-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83690-4-RR (MARS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00679-1

    Sandwich ELISA standard curve of MP00679-1

    Sandwich ELISA standard curve of MP00679-1, Human MARS Recombinant Matched Antibody Pair - PBS only. 83690-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6619. 83690-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP00679-1

    Sample test of MP00679-1

    HepG2 and A549 cell lysates were measured. The human MARS concentration of detected samples was determined to be 139.60 ng/mL (based on a 3.00 mg/mL extract load) in HepG2 cell lysate and 85.10 ng/mL (based on a 3.00 mg/mL extract load) in A549 cell lysate.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MARS Recombinant antibody (83690-4-RR)

    IF Staining of HepG2 using 83690-4-RR (same clone as 83690-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MARS Recombinant antibody (83690-4-RR)

    IF Staining of HepG2 using 83690-4-RR (same clone as 83690-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83690-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00679-1

    Cytometric bead array standard curve of MP00679-1

    Cytometric bead array standard curve of MP00679-1, MARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83690-4-PBS. Detection antibody: 83690-3-PBS. Standard: Ag6619. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00679-2

    Cytometric bead array standard curve of MP00679-2

    Cytometric bead array standard curve of MP00679-2, MARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83690-4-PBS. Detection antibody: 83690-2-PBS. Standard: Ag6619. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83690-4-RR

    Affinity and Kinetic Characterization of 83690-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83690-4-RR against Human MARS were performed. The affinity constant is 1.90 nM.

Product Information

83690-4-PBS targets MARS as part of a matched antibody pair:

MP00679-1: 83690-4-PBS capture and 83690-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00679-2: 83690-4-PBS capture and 83690-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MARS fusion protein Ag6619
Full Name methionyl-tRNA synthetase
Calculated Molecular Weight 101 kDa
GenBank Accession NumberBC002384
Gene Symbol MARS
Gene ID (NCBI) 4141
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.