MARS Recombinant antibody

MARS Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240648G4

Cat no : 83690-4-RR

Synonyms

MARS1, METRS, Methionyl-tRNA synthetase, Methionine--tRNA ligase, cytoplasmic, EC:6.1.1.10

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Validation Data Gallery

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  WB analysis using 83690-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83690-4-RR (MARS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83690-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83690-4-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human stomach cancer using 83690-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83690-4-RR (MARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IF Staining of HepG2 using 83690-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 83690-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (83690-4-RR, Clone: 240648G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83690-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83690-4-RR against Human MARS were performed. The affinity constant is 1.90 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, A549 cells, PC-12 cells, mouse testis tissue, rat liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83690-4-RR is MARS Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MARS fusion protein Ag6619
• Full Name: methionyl-tRNA synthetase
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC002384
• Gene Symbol: MARS
• Gene ID (NCBI): 4141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The methionyl-tRNA synthetase (MARS) gene encodes cytoplasmic methionyl-tRNA synthetase (MetRS) responsible for catalyzing the ligation of methionine to tRNA. MetRS belongs to a family of aminoacyl-tRNA synthetases that play critical roles in protein biosynthesis by charging tRNAs with their cognate amino acids. MetRS overexpression was shown to be evident in human colon cancer patients. MetRS may thus be involved in oncogenic transformation. MetRS has been proven as a potential prognostic marker candidate for the clinical prognostic prediction of non-small-cell lung cancer (NSCLC) in patients. MetRS can also be used to detect intracellular oxidative stress and control protein synthesis under oxidative stress (PMID: 30271085, PMID: 34679529, PMID: 32404475). The calculated molecular weight of MetRS is 101 kDa.