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UFD1L Recombinant antibody

UFD1L Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230411A2

Cat no : 83669-1-RR

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using UFD1L Recombinant antibody (83669-1-RR)

    FC experiment of HEK-293 using 83669-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using UFD1L Recombinant antibody (83669-1-RR)

    FC experiment of HeLa using 83669-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83669-1-RR

    Affinity and Kinetic Characterization of 83669-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83669-1-RR against Human UFD1L were performed. The affinity constant is below 1 pM.

Tested Applications

Positive FC (Intra) detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83669-1-RR is UFD1L Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UFD1L fusion protein Ag0954
Full Name ubiquitin fusion degradation 1 like (yeast)
Calculated Molecular Weight 35 kDa
GenBank Accession NumberBC005087
Gene Symbol UFD1L
Gene ID (NCBI) 7353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for UFD1L antibody 83669-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols