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SOX4 Recombinant antibody, PBS Only (Capture)

SOX4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240625E3

Cat no : 83660-1-PBS

Synonyms

Transcription factor SOX-4, EVI16, CSS10, 240625E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX4 Recombinant antibody (83660-1-RR)

    WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00619-1

    Sample test of MP00619-1

    The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using SOX4 Recombinant antibody (83660-1-RR)

    FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SOX4 Recombinant antibody (83660-1-RR)

    FC experiment of HeLa using 83660-1-RR (same clone as 83660-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00619-2

    Cytometric bead array standard curve of MP00619-2

    Cytometric bead array standard curve of MP00619-2, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-2-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83660-1-RR

    Affinity and Kinetic Characterization of 83660-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

Product Information

83660-1-PBS targets SOX4 as part of a matched antibody pair:

MP00619-1: 83660-1-PBS capture and 83660-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00619-2: 83660-1-PBS capture and 83660-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SOX4 fusion protein Ag26583
Full Name SRY (sex determining region Y)-box 4
Calculated Molecular Weight 474 aa, 47 kDa
GenBank Accession NumberBC072668
Gene Symbol SOX4
Gene ID (NCBI) 6659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.