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GLIPR1L1 Recombinant antibody, PBS Only (Detector)

GLIPR1L1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240594D10

Cat no : 83584-3-PBS

Synonyms

UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using GLIPR1L1 Recombinant antibody (83584-3-RR)

    WB analysis of human testis using 83584-3-RR (same clone as 83584-3-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83584-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using GLIPR1L1 Recombinant antibody (83584-3-RR)

    WB analysis of mouse testis using 83584-3-RR (same clone as 83584-3-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83584-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using GLIPR1L1 Recombinant antibody (83584-3-RR)

    FC experiment of PC-3 using 83584-3-RR (same clone as 83584-3-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83584-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00557-1

    Cytometric bead array standard curve of MP00557-1

    Cytometric bead array standard curve of MP00557-1, GLIPR1L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83584-2-PBS. Detection antibody: 83584-3-PBS. Standard: Ag14852. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83584-3-RR

    Affinity and Kinetic Characterization of 83584-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

Product Information

83584-3-PBS targets GLIPR1L1 as part of a matched antibody pair:

MP00557-1: 83584-2-PBS capture and 83584-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GLIPR1L1 fusion protein Ag14852
Full Name GLI pathogenesis-related 1 like 1
Calculated Molecular Weight 242 aa, 27 kDa
GenBank Accession NumberBC014603
Gene Symbol GLIPR1L1
Gene ID (NCBI) 256710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.