GLIPR1L1 Recombinant antibody

GLIPR1L1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240594D10

Cat no : 83584-3-RR

Synonyms

UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972

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Validation Data Gallery

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  WB analysis of human testis using 83584-3-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 83584-3-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of PC-3 using 83584-3-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83584-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue
• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83584-3-RR is GLIPR1L1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GLIPR1L1 fusion protein Ag14852
• Full Name: GLI pathogenesis-related 1 like 1
• Calculated Molecular Weight: 242 aa, 27 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC014603
• Gene Symbol: GLIPR1L1
• Gene ID (NCBI): 256710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.