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LONP1 Recombinant antibody

LONP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240460G10

Cat no : 83552-2-RR

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LONP1 Recombinant antibody (83552-2-RR)

    WB analysis using 83552-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83552-2-RR (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-251 cells using LONP1 Recombinant antibody (83552-2-RR)

    FC experiment of U-251 using 83552-2-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-2-RR, Clone:240460G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83552-2-RR

    Affinity and Kinetic Characterization of 83552-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83552-2-RR against Human LONP1 were performed. The affinity constant is 1.76 nM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83552-2-RR is LONP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LONP1 fusion protein Ag7196
Full Name lon peptidase 1, mitochondrial
Calculated Molecular Weight 106 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Protocols

Product Specific Protocols
WB protocol for LONP1 antibody 83552-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols