LONP1 Recombinant antibody, PBS Only (Capture)

LONP1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

240460G10

Cat no : 83552-2-PBS

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

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Validation Data Gallery

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  WB analysis using 83552-2-RR (same clone as 83552-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83552-2-RR (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83552-2-RR (same clone as 83552-2-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-2-RR, Clone:240460G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00531-1

  Cytometric bead array standard curve of MP00531-1, LONP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83552-2-PBS. Detection antibody: 83552-1-PBS. Standard: Ag7196. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 83552-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83552-2-RR against Human LONP1 were performed. The affinity constant is 1.76 nM.

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Product Information

83552-2-PBS targets LONP1 as part of a matched antibody pair:

MP00531-1: 83552-2-PBS capture and 83552-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LONP1 fusion protein Ag7196
• Full Name: lon peptidase 1, mitochondrial
• Calculated Molecular Weight: 106 kDa
• GenBank Accession Number: BC000235
• Gene Symbol: LONP1
• Gene ID (NCBI): 9361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.