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  • Western Blot (WB) analysis of various lysates using PSMD1 Recombinant antibody (83521-2-RR)

    WB analysis using 83521-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83521-2-RR (PSMD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PSMD1 Recombinant antibody (83521-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-2-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PSMD1 Recombinant antibody (83521-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83521-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-2-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of MCF-7 cells using PSMD1 Recombinant antibody (83521-2-RR)

    FC experiment of MCF-7 using 83521-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PSMD1 Recombinant antibody (83521-2-RR, Clone:240458B3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83521-2-RR

    Affinity and Kinetic Characterization of 83521-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83521-2-RR against Human PSMD1 were performed. The affinity constant is below 1 pM.

PSMD1 Recombinant antibody

PSMD1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83521-2-RR
Clone No.240458B3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458B3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inJurkat cells, SKOV-3 cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83521-2-RR targets PSMD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMD1 fusion protein Ag12009 Predict reactive species
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Calculated Molecular Weight 953 aa, 106 kDa
Observed Molecular Weight106 kDa
GenBank Accession NumberBC094720
Gene Symbol PSMD1
Gene ID (NCBI) 5707
RRIDAB_3671147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.

Protocols

Product Specific Protocols
WB protocol for PSMD1 antibody 83521-2-RRDownload protocol
IHC protocol for PSMD1 antibody 83521-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols