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SMPD2 Recombinant antibody, PBS Only (Detector)

SMPD2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240400B5

Cat no : 83520-3-PBS

Synonyms

N-SMase, Neutral sphingomyelinase, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, EC:3.1.4.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMPD2 Recombinant antibody (83520-3-RR)

    WB analysis using 83520-3-RR (same clone as 83520-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83520-3-RR (SMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using SMPD2 Recombinant antibody (83520-3-RR)

    FC experiment of U2OS using 83520-3-RR (same clone as 83520-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SMPD2 Recombinant antibody (83520-3-RR)

    FC experiment of HeLa using 83520-3-RR (same clone as 83520-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00518-1

    Cytometric bead array standard curve of MP00518-1

    Cytometric bead array standard curve of MP00518-1, SMPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83520-1-PBS. Detection antibody: 83520-3-PBS. Standard: Ag7367. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83520-3-RR

    Affinity and Kinetic Characterization of 83520-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83520-3-RR against Human SMPD2 were performed. The affinity constant is below 1 pM.

Product Information

83520-3-PBS targets SMPD2 as part of a matched antibody pair:

MP00518-1: 83520-1-PBS capture and 83520-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMPD2 fusion protein Ag7367
Full Name sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC000038
Gene Symbol SMPD2
Gene ID (NCBI) 6610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.