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SMPD2 Recombinant antibody

SMPD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240400B5

Cat no : 83520-3-RR

Synonyms

N-SMase, Neutral sphingomyelinase, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, EC:3.1.4.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMPD2 Recombinant antibody (83520-3-RR)

    WB analysis using 83520-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83520-3-RR (SMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using SMPD2 Recombinant antibody (83520-3-RR)

    FC experiment of U2OS using 83520-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using SMPD2 Recombinant antibody (83520-3-RR)

    FC experiment of HeLa using 83520-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83520-3-RR

    Affinity and Kinetic Characterization of 83520-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83520-3-RR against Human SMPD2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHepG2 cells, HEK-293T cells, MOLT-4 cells, SH-SY5Y cells
Positive FC (Intra) detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83520-3-RR is SMPD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMPD2 fusion protein Ag7367
Full Name sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC000038
Gene Symbol SMPD2
Gene ID (NCBI) 6610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).

Protocols

Product Specific Protocols
WB protocol for SMPD2 antibody 83520-3-RRDownload protocol
FC protocol for SMPD2 antibody 83520-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols