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TFF1 Recombinant antibody, PBS Only (Capture)

TFF1 Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240424F5

Cat no : 83467-2-PBS

Synonyms

Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using TFF1 Recombinant antibody (83467-2-RR)

    FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83467-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00469-1

    Cytometric bead array standard curve of MP00469-1

    Cytometric bead array standard curve of MP00469-1, TFF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83467-2-PBS. Detection antibody: 83467-1-PBS. Standard: Ag4541. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83467-2-RR

    Affinity and Kinetic Characterization of 83467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

Product Information

83467-2-PBS targets TFF1 as part of a matched antibody pair:

MP00469-1: 83467-2-PBS capture and 83467-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFF1 fusion protein Ag4541
Full Name trefoil factor 1
Calculated Molecular Weight 84 aa, 9 kDa
GenBank Accession NumberBC032811
Gene Symbol TFF1
Gene ID (NCBI) 7031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TFF1 (formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.