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  • Flow cytometry (FC) experiment of MCF-7 cells using TFF1 Recombinant antibody (83467-2-RR)

    FC experiment of MCF-7 using 83467-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83467-2-RR

    Affinity and Kinetic Characterization of 83467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

TFF1 Recombinant antibody

TFF1 Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 83467-2-RR
Clone No.240424F5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83467-2-RR targets TFF1 in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFF1 fusion protein Ag4541 Predict reactive species
Full Name trefoil factor 1
Calculated Molecular Weight 84 aa, 9 kDa
GenBank Accession NumberBC032811
Gene Symbol TFF1
Gene ID (NCBI) 7031
RRIDAB_3671097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04155
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

Protocols

Product Specific Protocols
FC protocol for TFF1 antibody 83467-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols