TREM2 Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP00440-4, Human TREM2 Monoclonal Matched Antibody Pair - PBS only. 83438-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0747. 83438-7-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL.

THP-1 cells (3 x 10^6 cells/mL) were cultured in RPMI with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernatants were removed and assayed for levels of human TREM2 and measured 1,626.6 pg/mL.

The mean human TREM2 concentration was determined to be 5,362.2 pg/mL in THP-1 cell extract based on a 3.0 mg/mL extract load.

Serum of sixteen individual healthy human donors were measured. The human TREM2 concentration of detected samples was determined to be 2,096.2 pg/mL with a range of 661.9-6,705.0 pg/mL.