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THOC5 Recombinant antibody

THOC5 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240013F7

Cat no : 83435-1-RR

Synonyms

KIAA0983, Functional spliceosome-associated protein 79, C22orf19, 240013F7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using THOC5 Recombinant antibody (83435-1-RR)

    IF Staining of U2OS using 83435-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using THOC5 Recombinant antibody (83435-1-RR)

    IF Staining of U2OS using 83435-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using THOC5 antibody (83435-1-RR, Clone: 240013F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using THOC5 Recombinant antibody (83435-1-RR)

    FC experiment of U2OS using 83435-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thoc5 Recombinant Antibody (83435-1-RR, Clone:240013F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83435-1-RR is THOC5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen THOC5 fusion protein Ag6664
Full Name THO complex 5
Calculated Molecular Weight 79 kDa
GenBank Accession NumberBC003615
Gene Symbol THOC5
Gene ID (NCBI) 8563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for THOC5 antibody 83435-1-RRDownload protocol
FC protocol for THOC5 antibody 83435-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols