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MCP-1 Recombinant antibody

MCP-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240341G3

Cat no : 83370-2-RR

Synonyms

Ccl2, chemokine (C C motif) ligand 2, MCP 1, MCP-1, Scya2, Sigje

Validation Data Gallery

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  • Western Blot (WB) analysis of NR8383 cells using Ccl2 Recombinant antibody (83370-2-RR)

    WB analysis of NR8383 using 83370-2-RR

    Untreated, LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83370-2-RR (Ccl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83370-2-RR

    Affinity experiments of 83370-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83370-2-RR against Rat MCP-1 were performed. The affinity constant is 0.836 nM.

Tested Applications

Positive WB detected inLPS and Brefeldin A treated NR8383 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83370-2-RR is MCP-1 Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name chemokine (C-C motif) ligand 2
Calculated Molecular Weight16 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberNM_031530
Gene Symbol Ccl2
Gene ID (NCBI) 24770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Protocols

Product Specific Protocols
WB protocol for MCP-1 antibody 83370-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols