Product Information
83362-2-PBS targets NEDD9 as part of a matched antibody pair:
MP00378-2: 83362-2-PBS capture and 83362-4-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | NEDD9 fusion protein Ag34170 Predict reactive species |
| Full Name | neural precursor cell expressed, developmentally down-regulated 9 |
| Calculated Molecular Weight | 834 aa, 93 kDa |
| Observed Molecular Weight | 105 kDa, 115 kDa |
| GenBank Accession Number | BC040207 |
| Gene Symbol | NEDD9 |
| Gene ID (NCBI) | 4739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14511 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).
