NEDD9 Recombinant antibody

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  WB analysis using 83362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 83362-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 83362-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 83362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83362-2 against Human NEDD9 were performed. The affinity constant is 0.173 nM.

NEDD9 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83362-2-RR

Clone No.240310F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, U-251 cells, HepG2 cells, Raji cells, Jurkat cells
• Positive IF/ICC detected in: MCF-7 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83362-2-RR targets NEDD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEDD9 fusion protein Ag34170
• Full Name: neural precursor cell expressed, developmentally down-regulated 9
• Calculated Molecular Weight: 834 aa, 93 kDa
• Observed Molecular Weight: 105 kDa, 115 kDa
• GenBank Accession Number: BC040207
• Gene Symbol: NEDD9
• Gene ID (NCBI): 4739
• RRID: AB_3671016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14511
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).